Hi, i had been stucked here for hours now, gone through lots of same issues but doesn't seem to fix mine. here is my command and the error.
qiime dada2 denoise-single
--i-demultiplexed-seqs demux.qza
--p-trim-left-f 20
--p-trim-left-r 20
--p-trunc-len-f 295
--p-trunc-len-r 234
--o-representative-sequences rep-seqs-dada2.qza
--o-table table-dada2.qza
--o-denoising-stats stats-dada2.qza
and comes with these errors
(1/4?) no such option: --p-trim-left-f Did you mean --p-trim-left?
(2/4?) no such option: --p-trim-left-r Did you mean --p-trim-left?
(3/4?) no such option: --p-trunc-len-f Did you mean --p-trunc-len?
(4/4?) no such option: --p-trunc-len-r Did you mean --p-trunc-len?
i have carefully typed the command to make sure i use the appropriate dash lines, yet i get same errors. please any suggestion?
Hi @pyghost,
I made this post public as this is the preferred method for asking for help on the QIIME 2 forum instead of DMs.
The error message here is actually being really nice and suggesting the correct syntax.
Your syntax = --p-trim-left-f
, what it should be --p-trim-left
, notice the -f
differences here.
Now, I also noticed that you are providing reverse read parameters, however, your command is calling the denoise-single
action, which actually does not have any reverse parameters.
Assuming you have pared-end data, I recommend typing qiime dada2 denoise-paired --help
for the appropriate parameter syntax to use.
ok, great. sorry about that. Thanks for the assistance.
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.