I am currently running the following command:
qiime demux emp-single
And was given the following error message:
Plugin error from demux:
No sequences were mapped to samples. Check that your barcodes are in the correct orientation (see the rev_comp_barcodes and/or rev_comp_mapping_barcodes options). If barcodes are NOT Golay format set golay_error_correction to False.
I have received this message before but it was a few years ago and I can't figure out what I did to fix it. I tried utilizing the -p-no-golay-error-correction to no avail. Does anyone know what a potential fix is? I did experience an issue with the sequencing run where there was no Index file generated, but after speaking with Illumina, I fixed this issue and re-ran the analysis to receive the Index file on the Local Run Manager.
Any help would be super appreciated.