I am a new user of qiime and I have a problem with importing input data;
I have a separate file for each of my samples (i.e. demultiplexed) and the mapping file. However, the barcodes were trimmed and do not appear in neither of these. In similar topics the answers assume that there is a separate file containing the barcodes or that they appear in the mapping file.
In this case the barcodes were completely removed after sequencing and cannot be retrieved from the existing files.
So, the question is whether the barcodes required to create an artifact and continue in analysis? Is there a way to circumvent this problem?
Thanks in advance,