New to Qiime2 Mismatched sequence ids:

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Hmm! Sorry to hear that @kulandai1. Let’s dissect that error message:

'Mismatched sequence ids: BARCODE_HEADER_ID, FORWARD_HEADER_ID, and REVERSE_HEADER_ID'

So if I fill in this “formula”:

BARCODE_HEADER_ID = 'HISEQ:504:HCJ2CBCXY:2:1101:3521:3486'
FORWARD_HEADER_ID = 'HISEQ:504:HCJ2CBCXY:2:1101:2578:2121'
REVERSE_HEADER_ID = 'HISEQ:504:HCJ2CBCXY:2:1101:2578:2121'

Whoa! Something looks mighty strange with that barcode ID. Would you be able to share your emp-paired-end-sequences.qza and sample-metadata.tsv? If you are unable to publicly share feel free to send a DM. If you are unable to share your data at all, can you please rerun the command, with --verbose included, and copy all of the output?

Thanks! :t_rex:

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