Need help with DADA2 error

Hi @adixit,
Welcome to the forum!
The --p-trunc-len changes @timanix suggested is not the issue here as your value of 0 there simply means no truncating is happening, so in essence it is in fact the maximum length you could have, therefore maximum overlap.

I think the important error is this:

which according to a similar post here might be related to something going wrong with the core calculations. Can you try running your script again but manually put the # of threads in --p-n-threads instead of leaving it to be automatically detected?

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