Hi one and all.
I am new to Qiime2 and I have 16s rRNA fastq files and I am unable to import them successfully.
I made a directory named data1 inside the documents folder/directory of my computer. I put in it my 4 fastq files (my sequences demultiplexed pair-end) and the manifest (tsv) file. My code is the following:
qiime tools import
--output-path data1 \
My manifest file content is:
Sample-id forward-absolute-filepath reverse-absolute-filepath
KC $PWD/data1/KC_1.fastq $PWD/data1/KC_2.fastq
FO $PWD/data1/KC_1.fastq $PWD/data1/FO_2.fastq
Please help me. I am using Qiime2 2021.8. Many thanks in advance.