Nanopore Long Read Demultiplexed Full 16S rRNA Seq

[colinbrislawn (Colin J Brislawn)]

Hello Malek,

Welcome to the forums!

I found this thread, which has a lot of similar questions.

Is there a way to use files from Nanopore in Qiime2 ?

Hello! I want to use files from Nanopore machine in Qiime2. I have looked through some questions and answers, but they have been quite a while. Hence, I want to ask similar topic at the moment. --------------My all preparations are as below-------- What specific kit chemistry (what version of what kit)? : NEBNext Ultra II End Repair/dATailing Module, NEBNext Quick Ligation Module, NEBNext Blunt/TA Ligase Master Mix, NEBNext FFPE DNA Repair Mix MinION Mk1B Starter Pack Native Barcoding Kit …

One key issue is that NanoPore will produce long, low-quality, and variable-length reads.

Variable-length reads break most assumptions about amplicons

Let us know what you try next!
Colin