I have a data set for targeted sequencing (ITS). So far, I have completed my steps for importing the data, summarizing and visualizing it. Before proceeding with DADA2 I want to remove the barcode and primer sequences. The primers are ITS5 & ITS2.
After going through the forum posts I am a bit confused about the best way to do this.
What is the best approach if I have multiple barcode sequences to remove?
I will be very thankful if anyone can guide me in this.
I have attached my metadata file for your reference.METADATA.tsv (1.7 KB)