I’m following the MOCK validation section from the Fungal ITS analysis tutorial. I’m working with a set of ITS2 reads from Illumina Miseq run, and when I tried to validate my pipeline with the mock community used in that tutorial (mock-25), I found that my pipeline didn´t find any ITS2 sequence. Is there any mock community specific to ITS2 region?
My pipeline is based on that plugin: Q2-ITSxpress: A tutorial on a QIIME 2 plugin to trim ITS sequences and the error is found in that step:
qiime itsxpress trim-pair-output-unmerged --i-per-sample-sequences MOCK/UPSTREAM/paired-end-demux.qza --p-region **ITS2** --p-taxa F --p-threads 8 --o-trimmed MOCK/UPSTREAM/trimmed2.qza
Thanks a lot