Hi Everyone, In my dataset, besides my samples, we also have a Mock sample (with forward and reverse reads) in order to assess our sequencing error rates and some controls (PCR blanks, with forward and reverse reads) to address for any contamination that might have happened during the processing of…

Hi @fstudart ! [image] fstudart: Is it possible in Qiime2 to use our Mock sample to estimate sequencing errors? I'm not sure I follow your question, can you provide some additional detail about what it is that you are trying to do? Thanks! [image] fstudart: we are interested in removing …

QIIME 2 now supports filtering of FeatureData[Sequence]! Check out the release notes to learn more! :tada:

Hi Dillon, Just one more question: after removing the sequences found in my PCR blanks from table.qza, do I also have to remove the same sequences from rep-seqs.qza? if so, is that possible? Because, I'll use this file later on in the analysis to build the tree. Thanks very much, FS

Hi @fstudart ! [image] fstudart: do I also have to remove the same sequences from rep-seqs.qza? You could probably get away with not removing those features, but your phylogenetic tree will have some extra tips in it, which may or may not impact downstream analyses. [image] fstudart: if …

@fstudart , Just to follow up, methods to assess accuracy using mock communities are now available as new actions evaluate-composition and evaluate-seqs as of the 2017.11 release. These are designed with mock communities in mind, but could also be useful for testing simulated communities or other sa…

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