Mock Communities for Assessing Quality Control

[Nicholas_Bokulich]

Welcome to the QIIME 2 forum, @asbarros!

Could you please let us know:

1. what command you are running
2. the full error message

I am making some assumptions based on the information you have given, but I think the issue is that you are using evaluate-composition to compare the feature tables as they are (with unique hash IDs for each feature), but this action should be run on collapsed feature tables. See this tutorial for an example (the evaluate-composition example is near the bottom of the tutorial):

Fungal ITS analysis tutorial

Fungal ITS analysis, mock communities, and more fun NOTE: This tutorial was written in a QIIME 2 2018.11 environment. It is not guaranteed to work with earlier or later versions of QIIME 2. This tutorial was compiled as a working exercise for a QIIME 2 workshop in December 2018, and does not represent the only possible fungal ITS workflow with QIIME 2, or even a benchmarked protocol recommendation. See other tutorials, e.g., the q2-itsxpress tutorial for other fungal ITS analysis options in QI…

I am also a little confused about how you are creating your "expected" compositions. It looks like the feature IDs of your expected features also consist of hash IDs; how did you obtain those? Presumably the feature IDs for the expected composition should be the taxonomic lineages of the species you added to the community; hash IDs makes it seem like this is additional sequencing data.