Hello,
I am attempting to run alpha rarefaction and I am having issues with my metadata file. The code I am using along with the error message is below:
(qiime2-amplicon-2023.9) qiime diversity alpha-rarefaction
--i-table table.qza
--i-phylogeny rooted-tree.qza
--p-max-depth 4000
--m-metadata-file metadata.tsv
--o-visualization alpha-rarefaction.qzv
--verbose
Plugin error from diversity:
The following IDs are not present in the metadata: 'CT_FLB_001', 'MCM_APR_001', 'MCM_FEB_001_1', 'MCM_NOV_002_1', 'MD_HOU_001_1', 'MI_HLK_001_1'
I used Keemei to validate my metadata file, and it found no issues, so I am uncertain what could be wrong with the format.
metadata.tsv (228.0 KB)