I have fastqc files for pair-end sequences, so I have R1 and R2 files. I want to create my metadata file, however, I do not know how to extract the barcodes. My sequences look like this:
Is TCGACGTC+ATGCCACA my barcode? Why is separated by a "+"? I extract these sequences from R1 and I have more than one. It is not supposed to have only one barcode per sample?