Hello, I am analysing 16srrna from feces of pigs. V3-V4 region sequence were retrieved from the Sequencing company. Only Forward and Reverse sequences were provided. From that data, I import into qiime by the following command:
qiime tools import
then i can follow the moving picture tutorial until reach Alpha and beta diversity analysis
with the following command:
qiime diversity core-metrics-phylogenetic
however, I got the following error massage:
"There was an issue with loading the file sample-metadata1.tsv as metadata:
Metadata file path doesn't exist, or the path points to something other than a file. Please check that the path exists, has read permissions, and points to a regular file (not a directory): sample-metadata1.tsv
There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org
Find details on QIIME 2 metadata requirements here: Metadata in QIIME 2 — QIIME 2 2021.4.0 documentation"
sample-metadata1.tsv file I used consist only sample-id column. This file locate in the right directory and checked with Keemei.
How can I fix this promblem and go on?