Happy New Year to all QIIME 2 developers and the community!
When I applied PICRUSt2 to my ITS data, I didn't generate the 'ko_metagenome_out' folder ; only ec_ITS_counts.txt_metagenome_out folder was generated. Is this normal?
Additionally, does it make sense to use MetaCyc pathway abundance to predict ITS functional analysis instead of KEGG pathways (since I didn't generate ko folder to convert it to kegg abundance as I did for 16s)?
What's the difference between these two databases ( kegg and metacyc) when performing exploratory prediction analysis of ITS please?