Matching ASVs with 16s Sanger amplicons.

[SoilRotifer (Mike Robeson)]

@WeedCentipede

Small question tho, in order to assign the taxonomy with vsearch using the 16s isolates database, is there any database curation process to take into account?

Well, you could try out our really cool sequence curation plugin, RESCRIPt. You should be able to work through the tutorials linked there. It'll be a good starting point to see what you can do with sequence curation.

@WeedCentipede

maybe assign the taxonomy to the isolates through different methods and then join them

You can do just that! That is, you can assign taxonomy to

1. your isolate database
2. SILVA and/or GreenGenes (see the Data resources page)
3. perhaps append your isolate sequences to the NCBI RefSeq db?
4. any combination ...

Then you can pick the one that appears most reasonable to you, or you can run rescript merge-taxa to return a taxonomy from all of the different approaches listed above, given certain parameters... One example use case is outlined here.