hello sir,
I’m really glad to see the conversion of yours. The same problem perplexed me.
We had the same error: “Filepath on line 1 and column “forward-absolute-filepath” could not be found ( /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.2A_1.fq.gz) for sample “A1.2A”.”
To be honest, I had followed the step you gave, but still received the same error, so I hope can get some suggestions from you. Really thanks.
sample-id forward-absolute-filepath reverse-absolute-filepath
A1.1A /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.1A_1.fq.gz. /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.1A_2.fq.gz|
A1.2A. /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.2A_1.fq.gz. /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.2A_2.fq.gz|
Command:
qiime tools import
–type “SampleData[PairedEndSequencesWithQuality]”
–input-format PairedEndFastqManifestPhred33V2
–input-path ./00.RawData-mainfest6.tsv
–output-path ./demux_seqs.qza