manifest import trouble: file cannot be found

[11139 (鑫玥 张)]

hello sir,
I’m really glad to see the conversion of yours. The same problem perplexed me.
We had the same error: “Filepath on line 1 and column “forward-absolute-filepath” could not be found ( /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.2A_1.fq.gz) for sample “A1.2A”.”
To be honest, I had followed the step you gave, but still received the same error, so I hope can get some suggestions from you. Really thanks.

sample-id forward-absolute-filepath reverse-absolute-filepath
A1.1A /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.1A_1.fq.gz. /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.1A_2.fq.gz|
A1.2A. /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.2A_1.fq.gz. /Users/zxy/QIIME2/00.RawData/A1.1A/FDMP21H002706-1a_L1_A1.2A_2.fq.gz|

Command:
qiime tools import
–type “SampleData[PairedEndSequencesWithQuality]”
–input-format PairedEndFastqManifestPhred33V2
–input-path ./00.RawData-mainfest6.tsv
–output-path ./demux_seqs.qza

[ChrisKeefe]

It’s hard to tell what the issue is without seeing the original manifest file, but the Filepath could not be found error message seems to be pretty clear:

I suspect that either:

1. Your filepath on that line is misspelled
2. You have unnecessary punctuation included in the filepath name (your copy/paste example has a period at the end of the manifest filepath.)

The ls command should help you confirm the spelling of your filenames - make sure they’re all exactly the same in the manifest as they are in your computer’s filesystem.

Good luck, and let us know what you find!
CK

[11139 (鑫玥 张)]

Thank you so much!! The file path name had problem, it worked after revising. Thank you again!
Good luck!