After using Qiime for some time, I feel ready to take the big stepa nd move to Qiime2
As I normally start my pipeline with demultiplexed paired-end files, I am tryng to import such files into Qiime2. here’s my command line:
qiime tools import \ --type 'SampleData[PairedEndSequencesWithQuality]' \ --input-format CasavaOneEightSingleLanePerSampleDirFmt \ --input-path manifest.2.txt \ --output-path ./demux.pe.qza
Unfortunately I receive the following error message:
There was a problem importing manifest.2.txt:
manifest.2.txt is not a directory.
Can you please advice?
Thank you, and wish you Happy Holidays