There was a problem importing /home/qiime2/new_carriers/manifest:
/home/qiime2/new_carriers/manifest is not a(n) SingleEndFastqManifestPhred33V2 file:
'absolute-filepath' is not a column in the metadata. Available columns: 'forward-absolute-filepath', 'reverse-absolute-filepath'
But I have rechecked and my headers are 'forward-absolute-filepath', 'reverse-absolute-filepath' and well spaced with tab, but still it does not work.
Thanks for including all of your commands and the error message, that is very helpful!
I'd like to call out a specific line in your error message:
You've set the --input-format parameter to SingleEndFastqManifestPhred33V2 when it sounds like you are working with paired end reads (from your comment regarding 'forward-absolute-filepath' and 'reverse-absolute-filepath').
You can view the list of importable formats by typing in qiime tools import --show-importable-formats. From there, you should be able to find the appropriate format for your data - and you can re-run your import with the correct --input-format included.