There was a problem importing /home/qiime2/new_carriers/manifest:
/home/qiime2/new_carriers/manifest is not a(n) SingleEndFastqManifestPhred33V2 file:
'absolute-filepath' is not a column in the metadata. Available columns: 'forward-absolute-filepath', 'reverse-absolute-filepath'
But I have rechecked and my headers are 'forward-absolute-filepath', 'reverse-absolute-filepath' and well spaced with tab, but still it does not work.
thermokarst
(Matthew Ryan Dillon)
assigned lizgehret
#2
Thanks for including all of your commands and the error message, that is very helpful!
I'd like to call out a specific line in your error message:
You've set the --input-format parameter to SingleEndFastqManifestPhred33V2 when it sounds like you are working with paired end reads (from your comment regarding 'forward-absolute-filepath' and 'reverse-absolute-filepath').
You can view the list of importable formats by typing in qiime tools import --show-importable-formats. From there, you should be able to find the appropriate format for your data - and you can re-run your import with the correct --input-format included.