Manifest file error- Decoded Phred score is out of range [0, 62]

[linki11]

Hi, I am going to pair two end sequences after trimming. but I face problem in importing data. As I have many samples in one project, so I need to create a manifest file for pair two end sequences.
command I enter is

qiime tools import \
--type 'SampleData[PairedEndSequencesWithQuality]' \
--input-path /Users/linkilam/paried-ends/manifest.txt \
--input-format PairedEndFastqManifestPhred64V2 \
--output-path /Users/linkilam/paried-ends/imported_samples.qza

Then the error message is " UserWarning: Importing of PHRED 64 data is slow as it is converted internally to PHRED 33. Working with the imported data will not be slower than working with PHRED 33 data.
warnings.warn(_phred64_warning)" and " ValueError: Decoded Phred score is out of range [0, 62].

An unexpected error has occurred:

Decoded Phred score is out of range [0, 62].

See above for debug info."
how can I solve this problem ?

[colinbrislawn (Colin J Brislawn)]

Hello linki11,

This error is related to the Phred score used:

@linki11

Decoded Phred score is out of range [0, 62]

You could try using Phred33 instead:

--input-format PairedEndFastqManifestPhred33V2

Let us know what you try next and how it works!

---

P.S. I've edited your post to put your code in a code block

[linki11]

tried it. It worked.
Thank you for your kind help.