Hi @Alessandra_Dupont ,
I have not checked your manifest, but the error is not indicating a problem in the manifest file. The issue is that QIIME 2 cannot import bz2-archived sequences. See here:
Hi @Danilo_Reis ,
Welcome in the forum!
To answer straight to your question, if you prepare a manifest file for the importing step, you don’t need to move all files in the same folder, as long as you specify the path for each file correctly.
However, there are few things to note. I don’t think qiime2 is able to work with ‘bz2’ files, hence you may have to decompress (then recompress with gzip if you want to save space, you can import as ‘fastq.gz’ files).
On the files you need, you probably w…
good luck!
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