making sample-ids appear in a specific order in the heatmap

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Hi @anirban.mcgill!

Check out the --p-cluster option:

  --p-cluster TEXT Choices('samples', 'features', 'both', 'none')
                         Specify which axes to cluster.      [default: 'both']

You can disable sample-axis clustering, which will keep the samples in the same order they are provided in the table.

You should be able to use this method to convert to a feature table:

https://docs.qiime2.org/2020.6/plugins/available/sample-classifier/metatable/

Using data from the Moving Pictures tutorial:

qiime sample-classifier metatable \
  --i-table table.qza \
  --m-metadata-file core-metrics-results/evenness_vector.qza \
  --m-metadata-file core-metrics-results/faith_pd_vector.qza \
  --m-metadata-file core-metrics-results/observed_features_vector.qza \
  --m-metadata-file core-metrics-results/shannon_vector.qza \
  --o-converted-table merged-table.qza

qiime feature-table transpose \
  --i-table merged-table.qza \
  --o-transposed-feature-table transposed-table.qza

qiime feature-table filter-features \
  --i-table merged-table.qza \
  --m-metadata-file transposed-table.qza \
  --p-where "[id] IN ('pielou_evenness', 'faith_pd', 'observed_features', 'shannon_entropy')"\
  --o-filtered-table filtered-table.qza

qiime feature-table heatmap \
  --i-table filtered-table.qza \
  --p-cluster none \
  --o-visualization heatmap.qzv

image

Hope that helps! :qiime2:

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