Hello everyone,
I am new to QIIME2 so I apologize up front for a rudimentary question.
I would like to make a taxa-bar-plots.qzv from my dataset, AFTER all contaminant samples are removed. I removed all taxa from my contaminant sample (Quiagenkit) from my experimental samples in my ASV_taxo.tsv file; and then I removed the Quiagenkit sample completely from my map.
Here is the code I ran to produce the taxa-bar-plots.qzv:
--i-table DOC-table.qza
--i-taxonomy DOC-taxonomy.qza
--m-metadata-file DOC_map_contaminants_removed.tsv
--o-visualization taxa-bar-plots.qzv
Here is the error message it produced:
Plugin error from taxa:
Sample IDs found in the table are missing in the metadata: {'Qiagenkit'}.
Debug info has been saved to /var/folders/bg/x61stg5n6qqf7n16x12q0jjr0000gn/T/qiime2-q2cli-err-hbdvl6xt.log
When I repeated the code with my original map (including contaminants) it worked:
--i-table DOC-table.qza
--i-taxonomy DOC-taxonomy.qza
--m-metadata-file DOC_map.tsv
--o-visualization taxa-bar-plots.qzv
But again, I would like the bar plot to exclude my contaminants. How might I go about doing that?
Thanks so much,
Aurora
DOC_map_contaminants_removed.tsv (2.0 KB)
DOC_map.tsv (3.7 KB)
DOC_ASV_taxo_contaminants_removed.tsv (181.4 KB)