I have query regarding one of my fungal samples (ITS1&2 region sequenced sample). It culture report result was fusarium species. whereas QIIME taxonomy identification analysis showed Aspergillus flavus.
I took both ITS 1 and ITS2 forward and reverse reads of my fungal sample and ran individually as emp-single-end-sequences. If I do blast analysis of generated OTU reads of my fungal sample means Fusarium species was seen along with Aspergillus flavus. But, I couldn’t see fusarium species presence in taxaplot after adding that fusarium OTU seq in existing database too.
Herewith, I have attached screenshot of blast result as well as taxaplot for your perusal. kindly have a look on it.
is there any way to see that fusarium species along with A.flavus in taxaplot?
Could I report this problem like that in manuscript for this sample?
Thanking you in advance. Looking forward to your reply