ITS extraction error - Missing sequence for record beginning on line 17

ITSexpress 2.1.1. gave me same result :confused:

Plugin error from itsxpress:

/tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-rjolube4/X06T_ATGCCTAA_L001_R2_001.fastq.gz is not a(n) FastqGzFormat file:

Missing sequence for record beginning on line 17

Debug info has been saved to /tmp/qiime2-q2cli-err-bs8swblf.log

I am trying ITS express 2.1.4. (THX @Adam_Rivers ) now, and.... this also returns me same error :confused: Weird...

But kind a small progress here:
From all my data i tested ive got this same Err log. Nevertheles today I tried repeat the extraction step on tutoruial demux data from Fungal ITS analysis tutorial and this worked. Total confusion :smiley:

ir-output-unmerg(qiime2-amplicon-2023.9) svecka@svecserver:~/FRESH/FRESH_ITS/qiime2/tutotial$ qiime itsxpress trim-pair-output-unmerged
sample-s> --i-per-sample-sequences demux-trimmed.qza
on ITS> --p-region ITS1 \

--p-taxa F
--p-threads 60
--o-trimmed tutorial_ITS_extracted.qza
Saved SampleData[PairedEndSequencesWithQuality] to: tutorial_ITS_extracted.qza