ITS extraction error - Missing sequence for record beginning on line 17

[salias (Sergio Alías-Segura)]

Hello @Pardal_Oblackovy !

@Pardal_Oblackovy

X13T_AAGGTACA_L001_R1_001.fastq.gz is not a(n) FastqGzFormat file:

Missing sequence for record beginning on line 17

This suggests that you may have some corrupted read around line 17 on sample X13T forward reads. In this post a similar issue is addressed. Could you please run:

# If your FASTQ file is compressed
zcat path/to/fastq.gz | head -n 30

# Else
head path/to/fastq -n 30

and share the output with us? Maybe we can visually spot the issue

Best,

Sergio