Hey @Mehrbod_Estaki,
Thanks a lot for your sharing, it is very useful!
I also made it running with only biom file uploaded in bugbase. But when I added my metadata, it keeps fail. I really have no idea why this happened and I think my .txt file meets all the criteria (see below).
it would be very nice if you can help me out a little bit:), thank you very much in advance!
Cheers
Hi @jqli21,
Welcome to the forum!
I moved your post to a new thread.
Can you please provide us with a bit more information about your problem? It's hard to help troubleshoot if we don't know what the problem actually is 
We need some details like which QIIME 2 version you're using, how your biom file was made, what is the exact error you are getting from BugBase or QIIME 2 (not sure where you are getting the error from). What are the commands/parameters you are using etc.
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