Hi
I am new to QIIME 2, I am using qiime2-2019.1 on a ubuntu 18.04 virtual machin on a windows host
I have 9 fastq.gz files for 9 samples, each file has both R1 and R2 reads in the same fastq file:
Let me know if that works for you. If you have any other questions, feel free to post them here along with the commands you tried and their outputs and messages.
Hi @colinbrislawn
Thanks for your reply
I have gone through the tutorial before, I not sure how to make the path for the forward and reverse reads since I have forward and reverse reads in the same file, how to make like following option
sample-id,absolute-filepath,direction
Lines starting with '#' are ignored and can be used to create
Should I do it as a single end, but each sample id is appeared twice for R1 and R2?
then how to fix the menifest for the following files
sample_0_reads.fq.gz sample_4_reads.fq.gz sample_8_reads.fq.gz
sample_1_reads.fq.gz sample_5_reads.fq.gz sample_9_reads.fq.gz
sample_2_reads.fq.gz sample_6_reads.fq.gz
sample_3_reads.fq.gz sample_7_reads.fq.gz