UPDATE:
I can no longer respond to this thread but thought I'd update here! I was able to fix the problem by completely uninstalling Rstudio and R and then creating a new conda environment with the latest version of R! hope this helps anyone in the future!
Hi There,
I've seen this question on the forum before, and none of the answers seem to address the same situation I'm in.
Every time I run dada2, I get this error:
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
Debug info has been saved to /var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/qiime2-q2cli-err-phg0be4f.log
The log says this:
command: run_dada_paired.R /var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/forward /var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/reverse /var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/output.tsv.biom /var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/track.tsv /var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/filt_f /var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/filt_r 200 200 25 25 2.0 2.0 2 12 independent consensus 1.0 0 1000
R version 4.1.1 (2021-08-10)
Loading required package: Rcpp
Error: package or namespace load failed for ‘Rcpp’ in dyn.load(file, DLLpath = DLLpath, ...):
unable to load shared object '/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/R/library/Rcpp/libs/Rcpp.so':
dlopen(/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/R/library/Rcpp/libs/Rcpp.so, 6): Library not loaded: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libR.dylib
Referenced from: /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/R/library/Rcpp/libs/Rcpp.so
Reason: image not found
Error: package ‘Rcpp’ could not be loaded
Execution halted
Traceback (most recent call last):
File "/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/python3.8/site-packages/q2_dada2/_denoise.py", line 266, in denoise_paired
run_commands([cmd])
File "/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/python3.8/site-packages/q2_dada2/_denoise.py", line 36, in run_commands
subprocess.run(cmd, check=True)
File "/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/python3.8/subprocess.py", line 516, in run
raise CalledProcessError(retcode, process.args,
subprocess.CalledProcessError: Command '['run_dada_paired.R', '/var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/forward', '/var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/reverse', '/var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/output.tsv.biom', '/var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/track.tsv', '/var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/filt_f', '/var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/tmphfd5i8ov/filt_r', '200', '200', '25', '25', '2.0', '2.0', '2', '12', 'independent', 'consensus', '1.0', '0', '1000']' returned non-zero exit status 1.
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File "/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/commands.py", line 329, in __call__
results = action(**arguments)
File "<decorator-gen-514>", line 2, in denoise_paired
File "/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/sdk/action.py", line 244, in bound_callable
outputs = self._callable_executor_(scope, callable_args,
File "/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/sdk/action.py", line 390, in _callable_executor_
output_views = self._callable(**view_args)
File "/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/python3.8/site-packages/q2_dada2/_denoise.py", line 279, in denoise_paired
raise Exception("An error was encountered while running DADA2"
Exception: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
and just for more information, when I type env
I get:
S=x86_64-apple-darwin13.4.0-as
LDFLAGS=-Wl,-pie -Wl,-headerpad_max_install_names -Wl,-dead_strip_dylibs -Wl,-rpath,/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib -L/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib
AR=x86_64-apple-darwin13.4.0-ar
RSTUDIO_WHICH_R=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/R
ac_cv_func_malloc_0_nonnull=yes
JAVA_LD_LIBRARY_PATH=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/server
TERM_PROGRAM=Apple_Terminal
ac_cv_func_realloc_0_nonnull=yes
OBJC=x86_64-apple-darwin13.4.0-clang
HOST=x86_64-apple-darwin13.4.0
SHELL=/bin/bash
TERM=xterm-256color
NM=x86_64-apple-darwin13.4.0-nm
PYTHONNOUSERSITE=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/python*/site-packages/
CPPFLAGS=-D_FORTIFY_SOURCE=2 -isystem /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/include
TMPDIR=/var/folders/vq/myv7llhj7sdgmwrn1tt316br0000gn/T/
PAGESTUFF=x86_64-apple-darwin13.4.0-pagestuff
CONDA_SHLVL=3
LIPO=x86_64-apple-darwin13.4.0-lipo
CONDA_PROMPT_MODIFIER=(qiime2-2021.4)
TERM_PROGRAM_VERSION=433
SIZE=x86_64-apple-darwin13.4.0-size
NMEDIT=x86_64-apple-darwin13.4.0-nmedit
SDKROOT=/Library/Developer/CommandLineTools/SDKs/MacOSX.sdk
OLDPWD=/
TERM_SESSION_ID=256664C4-064C-4825-B026-A9AA8B2FE139
CXX_FOR_BUILD=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-clang++
INSTALL_NAME_TOOL=x86_64-apple-darwin13.4.0-install_name_tool
SEGEDIT=x86_64-apple-darwin13.4.0-segedit
GFORTRAN=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-gfortran
SEG_HACK=x86_64-apple-darwin13.4.0-seg_hack
USER=clairewilliams
CLANGXX=x86_64-apple-darwin13.4.0-clang++
LIBTOOL=x86_64-apple-darwin13.4.0-libtool
CONDA_TOOLCHAIN_BUILD=x86_64-apple-darwin13.4.0
FC_FOR_BUILD=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/-gfortran
CONDA_EXE=/usr/local/Caskroom/miniconda/base/bin/conda
OTOOL=x86_64-apple-darwin13.4.0-otool
SSH_AUTH_SOCK=/private/tmp/com.apple.launchd.1XBSJPCMdq/Listeners
DEBUG_FORTRANFLAGS=-march=core2 -mtune=haswell -ftree-vectorize -fPIC -fstack-protector -O2 -pipe -isystem /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/include -march=core2 -mtune=haswell -ftree-vectorize -fPIC -fstack-protector -O2 -pipe -Og -g -Wall -Wextra -fcheck=all -fbacktrace -fimplicit-none -fvar-tracking-assignments
CXXFLAGS=-march=core2 -mtune=haswell -mssse3 -ftree-vectorize -fPIC -fPIE -fstack-protector-strong -O2 -pipe -stdlib=libc++ -fvisibility-inlines-hidden -std=c++14 -fmessage-length=0 -isystem /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/include
STRINGS=x86_64-apple-darwin13.4.0-strings
CONDA_TOOLCHAIN_HOST=x86_64-apple-darwin13.4.0
_CE_CONDA=
CHECKSYMS=x86_64-apple-darwin13.4.0-checksyms
CONDA_PREFIX_1=/Users/clairewilliams/opt/miniconda2
PATH=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin:/usr/local/Caskroom/miniconda/base/bin:/Users/clairewilliams/opt/miniconda2/condabin:/usr/local/bin:/usr/bin:/bin:/usr/sbin:/sbin:/Library/Frameworks/Mono.framework/Versions/Current/Commands
MESON_ARGS=--buildtype release
CONDA_PREFIX_2=/usr/local/Caskroom/miniconda/base
DEBUG_CXXFLAGS=-march=core2 -mtune=haswell -mssse3 -ftree-vectorize -fPIC -fPIE -fstack-protector-strong -O2 -pipe -stdlib=libc++ -fvisibility-inlines-hidden -std=c++14 -fmessage-length=0 -Og -g -Wall -Wextra -isystem /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/include
BUILD=x86_64-apple-darwin13.4.0
LD=x86_64-apple-darwin13.4.0-ld
CONDA_PREFIX=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4
F90=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-gfortran
PWD=/Users/clairewilliams
JAVA_HOME=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4
STRIP=x86_64-apple-darwin13.4.0-strip
CMAKE_ARGS=-DCMAKE_AR=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-ar -DCMAKE_CXX_COMPILER_AR=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-ar -DCMAKE_C_COMPILER_AR=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-ar -DCMAKE_RANLIB=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-ranlib -DCMAKE_CXX_COMPILER_RANLIB=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-ranlib -DCMAKE_C_COMPILER_RANLIB=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-ranlib -DCMAKE_LINKER=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-ld -DCMAKE_STRIP=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-strip -DCMAKE_INSTALL_NAME_TOOL=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-install_name_tool -DCMAKE_LIBTOOL=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-libtool -DCMAKE_OSX_DEPLOYMENT_TARGET= -DCMAKE_BUILD_TYPE=Release -DCMAKE_OSX_SYSROOT=/Library/Developer/CommandLineTools/SDKs/MacOSX.sdk
MPLBACKEND=Agg
F95=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-gfortran
LANG=en_US.UTF-8
DEBUG_FFLAGS=-march=core2 -mtune=haswell -ftree-vectorize -fPIC -fstack-protector -O2 -pipe -isystem /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/include -march=core2 -mtune=haswell -ftree-vectorize -fPIC -fstack-protector -O2 -pipe -Og -g -Wall -Wextra -fcheck=all -fbacktrace -fimplicit-none -fvar-tracking-assignments
XPC_FLAGS=0x0
F77=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-gfortran
REDO_PREBINDING=x86_64-apple-darwin13.4.0-redo_prebinding
CXX=x86_64-apple-darwin13.4.0-clang++
CC_FOR_BUILD=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-clang
CLANG=x86_64-apple-darwin13.4.0-clang
_CE_M=
XPC_SERVICE_NAME=0
SHLVL=1
HOME=/Users/clairewilliams
FORTRANFLAGS=-march=core2 -mtune=haswell -ftree-vectorize -fPIC -fstack-protector -O2 -pipe -isystem /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/include
CFLAGS=-march=core2 -mtune=haswell -mssse3 -ftree-vectorize -fPIC -fPIE -fstack-protector-strong -O2 -pipe -isystem /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/include
_CONDA_PYTHON_SYSCONFIGDATA_NAME=_sysconfigdata_x86_64_apple_darwin13_4_0
FC=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-gfortran
CONDA_PYTHON_EXE=/usr/local/Caskroom/miniconda/base/bin/python
LOGNAME=clairewilliams
build_alias=x86_64-apple-darwin13.4.0
JAVA_HOME_CONDA_BACKUP=
CONDA_DEFAULT_ENV=qiime2-2021.4
DEBUG_CFLAGS=-march=core2 -mtune=haswell -mssse3 -ftree-vectorize -fPIC -fPIE -fstack-protector-strong -O2 -pipe -Og -g -Wall -Wextra -isystem /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/include
RANLIB=x86_64-apple-darwin13.4.0-ranlib
CMAKE_PREFIX_PATH=:/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4
CC=x86_64-apple-darwin13.4.0-clang
SEG_ADDR_TABLE=x86_64-apple-darwin13.4.0-seg_addr_table
host_alias=x86_64-apple-darwin13.4.0
JAVA_LD_LIBRARY_PATH_BACKUP=
LDFLAGS_LD=-pie -headerpad_max_install_names -dead_strip_dylibs -rpath /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib -L/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib
R_LIBS_USER=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/lib/R/library/
OBJC_FOR_BUILD=/usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/bin/x86_64-apple-darwin13.4.0-clang
FFLAGS=-march=core2 -mtune=haswell -ftree-vectorize -fPIC -fstack-protector -O2 -pipe -isystem /usr/local/Caskroom/miniconda/base/envs/qiime2-2021.4/include
_=/usr/bin/env
Any help you can provide would be greatly appreciated! Thanks so much!
Best,
Claire