Dear all,
I am using qiime2 and I am new to qiime (therefore I am doing the tutorial “Moving Pictures”).
I am blocked with the following command
qiime demux emp-single
–i-seqs emp-single-end-sequences.qza
–m-barcodes-file sample-metadata.tsv
–m-barcodes-column barcode-sequence
–o-per-sample-sequences demux.qza
–o-error-correction-details demux-details.qza
getting
There was an issue with loading the file sample-metadata.tsv as metadata:
Metadata file path doesn’t exist, or the path points to something other than a file. Please check that the path exists, has read permissions, and points to a regular file (not a directory): sample-metadata.tsv
There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org
Find details on QIIME 2 metadata requirements here: https://docs.qiime2.org/2020.2/tutorials/metadata/
the metadata file is the one downloaded from the tutorial where it says that the file contains errors but when I open the file with Keemei there are no errors…
It would be helpful to have the file without errors to be able to compare… because Keemei does not see any errors and qiime does not like the file.
maybe somebody can help
all the best
