issue with input to feature-table merge-seqs

[op9183]

Hi all!

Issue merging my data, code is as such:

qiime feature-table merge-seqs
--i-data rep-seqs-1.qza rep-seqs-2.qza
--o-merged-data merged-reps.qza

i've also tried splitting the inputs:

qiime feature-table merge-seqs
--i-data rep-seqs-1.qza
--i-data rep-seqs-2.qza
--o-merged-data merged-reps.qza

Error: Invalid vlue for '--i-data': received <FeatureData[Sequence], Phylogeny[Unrooted]> as an argument, which is incompatible with parameter: List[FeatureData[Sequence]]

Some context: Running qiime2/2024.2. I am merging data generated from dada2 denoising and put in the representative sequences output from that step, as suggested by the "Fecal microbiota transplant (FMT) study: an exercise" guide.

Mostly I'm just confused as to how it ended up being classified as an unrooted phylogeny type? How can I fix this?

Thanks!

[Oddant1 (Anthony Simard)]

Hello @op9183,

It looks like one of your rep-seqs.qza inputs is not what it seems. Can you please run qiime tools peek on rep-seqs-1.qza and on rep-seqs-2.qza and post the results here?

Thank you.

[op9183]

Oh I see! Fixed it Thanks so much!