Hello qiime2 community,
I was trying to assemble class weight for my samples. My sequences are v3-v4 sequences and qiita didn't seem much help there. So I followed this
But when I run
qiime clawback sequence-variants-from-samples --i-samples feature_table.qza --o-sequences sv.qza --verbose I get an error.
Plugin error from clawback:
Invalid characters in sequence: ['3', 'Z', 'o', 't', 'u'].
Valid characters: ['-', 'K', 'Y', 'N', 'D', 'R', 'H', '.', 'M', 'V', 'G', 'T', 'B', 'W', 'A', 'C', 'S']
lowercase if your sequence contains lowercase characters not in the sequence's alphabet.
See above for debug info.
However my OTU table is not generated by deblur/dada2 and hence cannot use
--p-no-hashed-feature flag which seems to be a solution to the error.
I would like to know if there is any other to run the command on my feature_table.qza (168.0 KB)
Thanks in advance.