I have species-level taxonomy assignments from the metaphlan/humann2 software, which are represented in QIIME2 as a FeatureTable[RelativeFrequency] artifact. Here, the values are fractions, and they sum to one for each sample.
Given that my data are not the sort of relative abundances discussed in the songbird paper (or anticipated by the q2-songbird plugin, which takes a FeatureTable[Frequency] as its input), is it appropriate or meaningful to use the songbird software on these data? If NOT, is it possible to transform them into some other form that could participate in the sort of differential ranking approach songbird employs to identify potentially interesting taxa? Or are there other methods more appropriate in this sort of data to identifying taxa that differ considerably between different conditions?
Any guidance would be greatly appreciated!
Amanda