Is it possible to convert fna files to Fastq fileS using qiime2 ?

[M_F (Meriem)]

Hello,
Is it possible to convert fna files to Fastq fileS using qiime2 ?

Thanks

[Nicholas_Bokulich]

Hi @M_F ,

No this is not possible — FASTQ files include both sequences + quality scores. fna files (==FASTA) only include sequence data, not quality scores. So you can convert fastq-->fasta, but not the other way around.

If you have quality scores in a separate file, then technically you can merge fasta + quality = fastq, but this is not something that QIIME 2 can do, since it is a very antiquated format to have a separate file of quality scores, very unusual these days.

Good luck!