Ion Torrent PGM, 16S rRNA dada2 denoise

[colinbrislawn (Colin J Brislawn)]

Hello Harry,

It shouldn't be crashing! How much RAM does your computer have? Which reference database are you using?

In this post, setting n-jobs to 1 is suggested for running dada2. How many jobs are you running?

How much RAM is needed for feature classification?

Just in addition to @Nicholas_Bokulich's comments, if you're worried about memory, don't set n-jobs to anything other than one. Most of the memory usage is in loading the classifier object, and the number of times you do that is n-jobs. For what it's worth, it runs just fine using greengenes on my MacOS laptop

Let us know how we can help,
Colin