Ion Torrent PGM, 16S rRNA dada2 denoise

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Hi Qiime 2 Team,

I am using dada2 to denoise my 16S rRNA seqs from PGM. My seqs have been pre-checked by mothur to screen out bad quality seqs. My seqs range from 300-400 bps, and I do not want to lose any base pairs.

  1. Could I just de-activate --p-trunc-len function by giving parameter 0?
  2. does dada2 require that all seqs are of the same length as deblur?
  3. If I set --p-trunc-len 350, will sequences shorter than 350 bp be deleted?

Thanks so much

Thanks

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