Hi to all,
I’m a very beginner in qiime2. I hope you could help me.
I have 4 fastaq files each one containig 48 samples, which have been sequentiated with Ion Torrent platform. I have gone through the forum and, as many people suggested, I would like to import my data by following the pipeline 'SampleData[JoinedSequencesWithQuality] with the source format SingleEndFastqManifestPhred33.
The problem is that I cannot construct a manifest file in which I put each sample below the column sample-id with the corresponding absolute filepath because I do not have a single fastaq file for each sample. So, I tried the same by putting each one of the 4 files I have below the column sample-id, each one with its corresponding format, but the format is not recognized by qiime.
Could someone give me some ideas on how to generate a proper manifest file in my case?
Thank you very much!