I have been having trouble with a step called exploring taxonomy for groups. I am currently trying to group samples by a metadata category by creating an average community using the following command line
qiime feature-table group --i-table /PATH/to/feature_table.qza --m-metadata-file /PATH/to/Metadata.txt --m-metadata-column USER-defined-Factor-Name --p-axis sample --p-mode mean-ceiling --o-grouped-table Grouped_feature_table.qza
My user defined factor name is SampleType.
However when I input all files I keep getting this error:
(1/1) Invalid value for '--m-metadata-file': There was an issue with
retrieving column 'sampletype' from the metadata.
I have checked over my metadata to ensure my sample id column was okay and it is. Ive searched up and down this forum and I have not found a specific solution to my problem. Someone please help.
I've attached my metadata here.
Cuttlefish_metadata.txt (213.5 KB)
Here is the deblur file Cuttlefish_OTUstable.qza (172.6 KB)
We are also using qiime2-2020.11 as we cannot update it to the current version because of the class. Thanks