November 16, 2022, 1:43am
Hello, I am running a Qiime2-2022.8 on a linux system. I have downloaded paired end fastq file (forward and reverse) from NCBI. I also created my manifest file which is in tsv and it attached to this message. My reads folder is in the directory /home/georgia/microbes/reads. Inside the reads folder i have my manifest file. Afterwards i tried running the following script:
qiime tools import
however when i run this, it keeps bringing the following error:
(1/1) Invalid value for '--input-path': Path 'gbemimanifest.tsv' does not
Please how can i resolve this. I tried following a thread on a similar issue but i couldn't
. Please help
November 16, 2022, 7:48am
Hello and welcome to the forum!
Looks like you have an extra "-" character in the file name.
November 16, 2022, 5:39pm
However, i encountered another problem when running deblur. it brought an error called plugin error from deblur: max() arg is an empty sequence after i ran the following code:
[email protected]:~/microbes/reads$ qiime deblur denoise-16S \
Attached to this message is a snapshot
November 16, 2022, 5:40pm
Thanks. I was able to resolve it
December 17, 2022, 11:40pm
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