Dear, I hope you are well. I am trying to do a taxonomic classification of my sequences using a pre-trained classifier (SILVA 138) that I downloaded from the Qiime2 page. I ran the following command:
qiime feature-classifier fit-classifier-naive-bayes
--i-reference-reads PE-ref-seqs.qza
--i-reference-taxonomy silva-138-99-tax-515-806.qza
--o-classifier silva-138-99-515-806-nb-classifier.qza
But I get this error:
Invalid value for '--i-reference-reads': 'PE-ref-seqs.qza' is not a
valid filepath
The three files are in the same folder, when verifying the PE-ref-seqs.qza file it is observed that it is a Qiime2 artifact. So I don't know where the error is, could you help me fix this?
Abjoint the images. I will be very grateful for your help.
Thank you very much for answering. I corrected the file name, and now I get this error:
Plugin error from feature-classifier:
not enough values to unpack (expected 2, got 0)
Debug info has been saved to /tmp/qiime2-q2cli-err-ve1_ej50.log