Dear, I hope you are well. I am trying to do a taxonomic classification of my sequences using a pre-trained classifier (SILVA 138) that I downloaded from the Qiime2 page. I ran the following command:
qiime feature-classifier fit-classifier-naive-bayes
But I get this error:
Invalid value for '--i-reference-reads': 'PE-ref-seqs.qza' is not a
The three files are in the same folder, when verifying the PE-ref-seqs.qza file it is observed that it is a Qiime2 artifact. So I don't know where the error is, could you help me fix this?
Abjoint the images. I will be very grateful for your help.
Your ls and validate shows:
Your feature-classifier command used the parameter:
Thank you very much for answering. I corrected the file name, and now I get this error:
Plugin error from feature-classifier:
not enough values to unpack (expected 2, got 0)
Debug info has been saved to /tmp/qiime2-q2cli-err-ve1_ej50.log
I'm not familiar with that error, but I found this response to a user who experienced the same error a few years ago: Plugin error from feature-classifier: not enough values to unpack (expected 2, got 0) - #9 by thermokarst
I'll let someone else respond to any further questions you may have, as I am not as familiar with this process as others on the forums.
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