Please read the following before posting!

*Hello, not exactly new. have used qiime2 just to repeat an analysis 6 months ago. Here I am trying to use it on a different laptop (unfortunately, windows). Again *

Step 1 I couldn't get the yaml using wget, so went to the website and downloaded it.

*Step 2: the document I was able to download was named *

*Step 3- *
conda env create -n qiime2-amplicon-2023.9 --file qiime2-amplicon-ubuntu-latest-conda.yml

This is the error I get. I had checked in the old forums and the trouble shooting didn't work for me.

I have been trying for three days.


Elapsed: -

An HTTP error occurred when trying to retrieve this URL.
HTTP errors are often intermittent, and a simple retry will get you on your way.

Hello Shwetha,

Welcome to the forums! :qiime2:

Thank you for your detailed explanation. These two things are related:

It looks like something is not working well with your internet connection.

Are you able to access from your laptop?
For me, it looks like this:

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Yes, I am confused too. So I removed everything and re-installed conda. I don't think this is woth qiime2 but more like the conda issue since I get this error for conda update conda, conda install panda..
I just was trying a bunch of stuff. I am getting the same error with the new installation. I have asked for a Mac and I am sincerely hoping this is not the same issue with Mac cause I need qiime2 for everythin in my lab. Do you have any alternative suggestion for me to use qiime2? Like a docker image?

Yes! Installing QIIME 2 using Docker — QIIME 2 2023.9.2 documentation

And here's a tutorial on Qiime2 and Docker: Running The Moving Pictures Tutorial In Docker (2018.11)

(Qiime2 is a great fit for Docker because it includes a lot dependencies and the devs have built a comprehensive integration and testing platform to make Docker images. :whale: )

EDIT: A mac will not solve your network problems, but the team that gives you computers can offer great advice! Ask them about installing conda packages and see what they say!

I use Qiime on Windows 10 with WSL. It works great! :window:

Thank you, I will give it a go. Hope things are smoother with Mac!


Hi, I just wanted to update. You were right, the WSL (Ubuntu) was not connecting to internet, hence the issue.. I had to update my etc/resolv.conf file. Thank you!

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