Input files for PICRUSt2

[rr220 (R)]

Hi,
When i run qiime2-PICRUSt, to run PICRUSt2 my input files are table-dada2.qza and rep-seqs-dada2.qza and run the following command which works:
qiime picrust2 full-pipeline
--i-table table-dada2.qza
--i-seq rep-seqs-dada2.qza
--output-dir picrust2_output
--p-hsp-method pic
Now I have received a project that is run by facility that has provided these output files:

1. CentroidInformation.fa.bz2,
2. Merges_Barcodes.fa.bz2
3. Merged_Reads.fa.bz2
4. OTU_Table.biom
5. OTU_Table.tre
   6.OTU_Table.tsv
   7.Raw_Reads1.fq.bz2
6. Raw_Reads3.fq.bz2
7. Raw_Reads2_barcodes.fq.bz2
   10.readQC

Which of these files would be the input for PICRUSt2 pipeline in the command I have mentioned above.

Best,
RR
`

[colinbrislawn (Colin J Brislawn)]

Hello again, RR,

Here's there list of third-party Qiime2 plugins (community developed plugins):
https://library.qiime2.org/plugins/

You will see q2-picrust2 on that list, and can follow the links to this picrust2 tutorial.

Your collaborator send you several fasta files, so it may be worth reaching out to them to learn what these are and choose the best one as the input to the --i-seq.

[rr220 (R)]

Thank you for your help.