I am new to all this. I have a fastq files containing multiplexed single-end reads obtained from an Ion torrent PGM machine. I also have a metadata txt file that seems to match the format requirements for using qiime2. My goal is to make OTU level analysis once the data is demultiplexed and cleaned.
However I have been incapable of importing the data.
After looking the moving pictures tutorial (and also checking that it ran in my computer) and reading several forums, I haven’t been able to even import the files. The last command I used was:
qiime demux emp-single
The error that showed this time is this:
(1/1) Invalid value for “–i-seqs”: ‘Manifest1.txt’ is not a QIIME 2 Artifact
If I first try to import the manifest file with qiime import:
qiime tools import \
–type ‘SampleData[SequenceWithQuality]’ \
–input-path Manifest1.txt \
–output-path ION1-mux.qza \
this error appears:
An unexpected error has occurred:
Semantic type SampleData[SequenceWithQuality] does not have a compatible directory format.
See above for debug info.
Im not sure what I must do and I ran out of ideas.