Importing sequence files (.fna) files

[Nicholas_Bokulich]

@divyaprince321

Again error but this time a long error saying can not allocate memory.
I think I might be out of memory besides allocating 10.2gb memory on vbox.

That is correct. You can search the forum archive to see how others have addressed this same error. Unfortunately, allocating more memory to the virtual box and using a more powerful computer may be your only options.

@divyaprince321

Now my final request is I need a relative abundance table of the otus and the representative sequence file.
If I will run closed reference command
Will I get these two files

The help documentation for all QIIME 2 actions details the outputs and their content. E.g., for this action type this into the terminal and hit enter:

qiime vsearch cluster-features-closed-reference --help

Among other details, you will see this:

Outputs:
  --o-clustered-table ARTIFACT FeatureTable[Frequency]
                          The table following clustering of features.
                                                                    [required]
  --o-clustered-sequences ARTIFACT FeatureData[Sequence]
                          The sequences representing clustered features,
                          relabeled by the reference IDs.           [required]
  --o-unmatched-sequences ARTIFACT FeatureData[Sequence]
                          The sequences which failed to match any reference
                          sequences. This output maps to vsearch's
                          --notmatched parameter.                   [required]

@divyaprince321

Secondly which file I have to use as reference-sequences in case of picking closed reference otus.

Your reference sequences of choice, imported as a FeatureData[Sequence] artifact. The data resources at qiime2.org contain several pre-formatted examples.

Good luck!