importing paired FASTQ file into qiime had errors. any help?

[HebaHussein-1981 (Heba Hussein)]

wow we both had the same idea

[ben (benjamin w.)]

No this was my fault, I didn't look up what we needed to name the columns in the manifest. Ben

[HebaHussein-1981 (Heba Hussein)]

at least it started to read files now. That is a progress

[HebaHussein-1981 (Heba Hussein)]

we did it. it started running but there was a bugFile "//miniconda3/envs/qiime2-2019.7/lib/python3.6/site-packages/skbio/io/registry.py", line 506, in read
return (x for x in itertools.chain([next(gen)], gen))
File "//miniconda3/envs/qiime2-2019.7/lib/python3.6/site-packages/skbio/io/registry.py", line 531, in _read_gen
yield from reader(file, **kwargs)
File "//miniconda3/envs/qiime2-2019.7/lib/python3.6/site-packages/skbio/io/registry.py", line 1008, in wrapped_reader
yield from reader_function(fhs[-1], **kwargs)
File "//miniconda3/envs/qiime2-2019.7/lib/python3.6/site-packages/skbio/io/format/fastq.py", line 354, in _fastq_to_generator
qual_header)
File "//miniconda3/envs/qiime2-2019.7/lib/python3.6/site-packages/skbio/io/format/fastq.py", line 523, in _parse_quality_scores
phred_offset=phred_offset))
File "//miniconda3/envs/qiime2-2019.7/lib/python3.6/site-packages/skbio/io/format/_base.py", line 35, in _decode_qual_to_phred
% (phred_range[0], phred_range[1]))
ValueError: Decoded Phred score is out of range [0, 62].

An unexpected error has occurred:

Decoded Phred score is out of range [0, 62].

See above for debug info.

[ben (benjamin w.)]

Change PairedEndFastqManifestPhred64V2 to

PairedEndFastqManifestPhred33V2

Then rerun

[HebaHussein-1981 (Heba Hussein)]

wooow we did it

[HebaHussein-1981 (Heba Hussein)]

thanks a lot for your help. now how to visualize?
I am excited to see it

[ben (benjamin w.)]

That, you're going to have to dig around a bit, I have a procedure to do tomorrow AM so I must sleep. Ben

[HebaHussein-1981 (Heba Hussein)]

thanks a lot for your help. At least we imported the file. I will do the search