Importing my data to qiime

User Support
1

Hi everyone, I've tried importing my data, but the problem persists. I would appreciate your help, please.

$ qiime tools import
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path manifest.tsv
--output-path results/demux-paired.qza
--input-format PairedEndFastqManifestPhred33V2
There was a problem importing manifest.tsv:

manifest.tsv is not a(n) PairedEndFastqManifestPhred33V2 file:

There was an issue with loading the metadata file:

Metadata IDs must be unique. The following IDs are duplicated: 'ACAP1', 'ACAP2', 'ACAP3', 'ACHP1', 'ACHP2', 'ACHP3', 'ACMP1', 'ACMP2', 'ACMP3', 'APAP2', 'APAP3', 'APHP1', 'APHP2', 'APHP3', 'APMP1', 'APMP2', 'APMP3'

There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org

Find details on QIIME 2 metadata requirements here: Metadata in QIIME 2 — QIIME 2 2024.10.1 documentation

2

Hello Sheys,

Welcome to the forums! :qiime2:

Can you upload and share your manifest.tsv file? If not, that's okay...

I can help you look for these duplicate IDs!

3

Thank you, the problem was the lines. My manifest.tsv had repetitions in the lines and should be R1 and R2 on the same line. It’s done :smiley:

Thank you.