I have 10 fastq files. 5 fwd,5 rev. and I excel file containing barcode sequence for fwd and reverse files. basically i have 50 gut samples from different regions. I want to import my data to qiime2. but i m unable to do it. If anyone could help me in this regard
Can you tell us what you have tried with regards to importing and what has failed for you? What were the errors?
This seems like a pretty simple paired-end multiplex impor process x 5 (once for each run)
I don’t have barcodes.fastq file.
As long as the barcodes are in your sequences (and not in the headers of the .fastq files) you can use the cutadapt demux-paired plugin to demultiplex your reads using a metadata file. In your case the excel file. Give that a go and let us know if you run into any problems.