Importing metadata file

[dr.deepakkukkar (Deepak Kukkar)]

Dear all,

I am facing a very strange issue while importing sample metadata file. I rund the following command:
qiime tools import \

--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path /mnt/d/Skill_enhancement/QIIME2/manifest.tsv
--input-format PairedEndFastqManifestPhred33V2
--output-path /mnt/d/Skill_enhancement/QIIME2/paired-end-demux.qza

The error message is as follows:

There was a problem importing /mnt/d/Skill_enhancement/QIIME2/manifest.tsv:

/mnt/d/Skill_enhancement/QIIME2/manifest.tsv is not a(n) PairedEndFastqManifestPhred33V2 file:

manifest.tsv (175 Bytes)
'forward-absolute-filepath' is not a column in the metadata. Available columns: 'absolute-filepath', 'direction'

I have uploaded my metadata file here. So can anyone please check what are the possible reasons for my errors and how to trouble shoot them? I have tried everything to the best of my abilities but still getting errors.

Thanks and regards,

Deepak

[colinbrislawn (Colin J Brislawn)]

Hello Deepak,

This formt PairedEndSequencesWithQuality requires different columns in the metadata.tsv file.

Here is what you have:

sample-id	absolute-filepath	direction
samplea	/mnt/d/Skill_enhancement/QIIME2/SRR17327077_1.fastq	forward
sampleb	/mnt/d/Skill_enhancement/QIIME2/SRR17327077_2.fastq	reverse

Notice how the column 'forward-absolute-filepath' is missing, just like the error says.

[dr.deepakkukkar (Deepak Kukkar)]

Hello Brislawn.

Thank you so much for your reply. I relabled the 2nd column header as 'forward-absolute-file-path', and also relabeled the 3rd column as reverse-absolute-filepath to modify my manifest file. Finally, I made a few more tweaks in the manifest file to import the data successfully. I am attaching the file here as well for other members of the community as well. I hope it helps. Deepak
manifest.tsv (174 Bytes)

[colinbrislawn (Colin J Brislawn)]

Great!

Were you able to import the data with this new manifest file?
(If you get an new error, you can post that here!)

EDIT: I found your new post about DADA2. Thank you for making a new thread!