Importing format with multiplexed fastq format, single read iwth barcode data in the reads, both forward and reverse directions

Hi @hrv20! We don’t have direct support for importing this data format, though there’s an open issue tracking progress on it. You might also check out this forum topic for some ideas from @Nicholas_Bokulich. We’ll follow up here when we have direct support for this data format in a QIIME 2 release!

In the meantime, you could try getting the reads into the same orientation such that every sequence begins with a barcode, and then import that data and demultiplex it following the q2-cutadapt Community Tutorial.